Regulatory

Part:BBa_K2560302:Design

Designed by: Benjamin Daniel   Group: iGEM18_Marburg   (2018-10-06)


Malonyl-CoA Sensor. This part is a PhytoBrick variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The j23100 was placed in a way to be so close to the fapO regulator as to be obstructed by the binding of the FapR protein


Source

One part of this part was constructed from the andersen promoter J23100 and the fap0 regulator binding site from the B. subtilis 168 genome.

References